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The ASD children also had more immediate adverse reactions to vaccinations (RI = 2.5, p = 0.002)

Quality assessment The methodological quality of randomized controlled trials ( n = 4) was assessed independently by two reviewers using the Cochrane Risk of Bias tool version 2 (RoB 2)

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For better accuracy, we also used unstained proteins as molecular weight markers, detected after transfer to PVDF by Ponceau Red staining (not shown)